| 基于计算机模拟的蛋白质分离纯化实验 |
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| 引用本文： | 刘文彬,张鹏,占磊,何平. 基于计算机模拟的蛋白质分离纯化实验[J]. 生物数学学报, 2010, 0(3): 493-500 |
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| 作者姓名： | 刘文彬 张鹏 占磊 何平 |
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| 作者单位： | 华南农业大学生命科学学院,广东广州510642 |
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| 基金项目： | 广东省自然科学基金项目（7006655）; 广东省科技计划项目（2008B011000006）; 国家自然科学基金项目（50977041） |
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| 摘 要： | 利用数学建模的方法首次提出了衡量分离纯化效果优劣的定量指标——纯化效益;建立了便于应用推广的聚乙二醇(polyethyleneglycol,PEG)和硫酸铵沉淀分离纯化模型,并以海芋过氧化物酶分离纯化为例,对模型进行求解、应用和检验,求解最优实验试剂用量.
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| 关 键 词： | 纯化效益 计算机模拟 过氧化物酶 |
Experiment of Protein Purification Based on Computer Simulation |
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| LIU Wen-bin,ZHANG peng ZHAN Lei,HE Ping. Experiment of Protein Purification Based on Computer Simulation[J]. Journal of Biomathematics, 2010, 0(3): 493-500 |
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| Authors: | LIU Wen-bin ZHANG peng ZHAN Lei HE Ping |
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| Affiliation: | (College of Life Sciences,South China Agricultural University,Guangzhou Guangdong 510642 China) |
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| Abstract: | In this paper,we created the concept of effectiveness of purification to measure the quality of the protein purification by the mathematical modeling methods;extablished the protein purification model of polyethylene glycol and ammonium sulfate that easily to be used; and applied them on the purification of the Alocasia peroxidase,chacking the model,to find out the best amount of the reagents used in the experiment. |
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| Keywords: | Effectiveness of purification Computer simulation Peroxidase |
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