Expression of the gene for large subunit of m-calpain is elevated in skeletal muscle from Duchenne muscular dystrophy patients |
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Authors: | Tajamul Hussain Harleen Mangath C Sundaram M P J S Anandaraj |
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Institution: | (1) Institute of Genetics, Begumpet, 500016 Hyderabad, India;(2) Nizam’s Institute of Medical Sciences, Punjagutta, 500 082 Hyderabad, India;(3) Present address: Centre for DNA Fingerprinting and Diagnostics, Nacharam, 500076 Hyderabad, India |
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Abstract: | Calpain is an intracellular nonlysosomal protease involved in essential regulatory or processing functions of the cell, mediated
by physiological concentrations of Ca2+. However, in an environment of abnormal intracellular calcium, such as that seen in Duchenne muscular dystrophy (DMD), calpain
is suggested to cause degeneration of muscle owing to enhanced activity. To test whether the reported increase in calpain
activity in DMD results fromde novo synthesis of the protease, we have assessed the quantitative changes in mRNA specific for m-calpain. mRNA isolated from DMD
and control muscle was analysed by dot blot hybridization using a cDNA probe for the large subunit of m-calpain. Compared
to control a four-fold increase in specific mRNA was observed in dystrophic muscle. This enhanced expression of the m-calpain
gene in dystrophic condition suggests that the reported increase in m-calpain activity results fromde novo synthesis of protease and underlines the important role of m-calpain in DMD. |
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Keywords: | m-calpain Duchenne muscular dystrophy muscle tissue RNA dot blot calpain genetics |
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