| 圆瓣姜花种子胚的组织培养与快速繁殖 |
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| 引用本文： | 熊友华,马国华,刘念,黄邦海.圆瓣姜花种子胚的组织培养与快速繁殖[J].广西植物,2005,25(3):241-244,i008. |
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| 作者姓名： | 熊友华 马国华 刘念 黄邦海 |
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| 作者单位： | 中国科学院华南植物园,广东,广州,510650;仲恺农业技术学院,广东,广州,510225;广州市农业技术推广中心,广东,广州,510520 |
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| 基金项目： | 广东省科技项目(20037C201029) |
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| 摘 要： | 采用广西百色地区野生圆瓣姜花的种子胚作外植体,成功地进行了离体组织培养与快速繁殖,建立了高频率的丛芽繁殖体系。实验结果表明:诱导种子胚萌芽的最适培养基为MS附加1.0mg·L16BA和0.2mg·L1NAA;在MS+6BA4.0mg·L1+NAA0.2mg·L1的培养基上最有利于诱导丛芽及增殖,芽的增殖系数达到5.28;1/2MS+IBA0.5mg·L1培养基最易诱导生根,生根率达97.7%。
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| 关 键 词： | 圆瓣姜花 种子胚 组织培养 速繁殖 |
| 文章编号： | 1000-3142(2005)03-0241-04 |
Tissue culture and rapid propagation of zygotic embryo of Hedychium forrestii |
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| XIONG You-hua,MA Guo-hua,LIU Nian,HUANG Bang-hai.Tissue culture and rapid propagation of zygotic embryo of Hedychium forrestii[J].Guihaia,2005,25(3):241-244,i008. |
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| Authors: | XIONG You-hua MA Guo-hua LIU Nian HUANG Bang-hai |
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| Institution: | XIONG You-hua1,MA Guo-hua1,LIU Nian 2*,HUANG Bang-hai3 |
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| Abstract: | Tissue culture of Hedychium forrestii was studied,using the zygotic embryo as explants. The results were as follows:The suitable condition for germination of zygotic embryo was in MS supplemented with 1.0 mg·L -1 6-BA and 0.2 mg·L -1 NAA;The best medium for propagation of multiple-shoot was MS+6-BA 4.0 mg·L -1+NAA 0.2 mg·L -1;1/2MS+0.5 mg·L -1 IBA could effectively promote the root emergence of the shoots,and the percentage of the root emergence accounted for 97.7%;The medium for transplanting was the mixture of perlite and peat(1∶1). |
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| Keywords: | Hedychium forrestii zygotic embryo tissue culture rapid propagation |
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