荷兰芹胚性愈伤组织诱导及胚状体发生与内源IAA和ABA关系的初步研究

分析了荷兰芹胚性愈伤组织发生的条件 ,对Co2 +作用下胚状体形成与培养物内源IAA和ABA的关系做了研究。结果表明 ,荷兰芹下胚轴胚性发生能力随其相对位置而异 ,自下而上逐渐提高。提高 2 ,4-D浓度有利于胚性愈伤组织的诱导 ,水解酪蛋白对胚性能力的表达没有显著影响。在胚状体发生过程中 ,添加Co2 +显著降低培养物内源IAA和ABA水平 ,并提高胚状体诱导率。其中对照组IAA有一个峰值 ,ABA有两个峰值 ;实验组ABA变化趋势与对照组相似 ,而IAA则始终没有峰值出现。Co2 +对IAA和ABA的抑制机制可能有所不同。

INDUCTION OF EMBRYONIC CALLUS AND FORMATION OF EMBRYOID RELATED TO ENDOGENOUS IAA AND ABA IN CELERY

Using hypocotyl of sterile celery seedling,induce conditions of embryonic callus were studied in this paper. Embryoid was obtained from the embryonic callus, and effect of Co 2+ on formation of the embryoid and levels of endogenous IAA and ABA were studied. The results showed that the competence of formation of the callus and embryonic callus from hypocotyl of celery decreased with its relative position from leaf end to apex of root. 2,4-D was favored to induction of embryonic callus. There was no significant effect of casein hydrolysate on expression of embryonic competence in celery. Endogenous levels of IAA and ABA in the cultures were reduced and induce rates of embryoid was increased by Co 2+ during embryoid formation of celery. There were one peak value of IAA levels and two peak values of ABA levels in the culture of control groups. The change of ABA levels in the culture of treatment groups was simular to control,but there was not peak value of IAA in the treatment. It was infered that there were difference effects Co 2+ inhibitation mechanism on between IAA and ABA in the formation of celery embryoid.