| Correlation between inhibition of calcium—dependent apoptosis by cyclosporin A and calcium transportation in HL—60 cells |
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| 引用本文： | HUANGQIQING MINGFANG 等.Correlation between inhibition of calcium—dependent apoptosis by cyclosporin A and calcium transportation in HL—60 cells[J].细胞研究,1996,6(1):23-30. |
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| 作者姓名： | HUANGQIQING MINGFANG |
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| 作者单位： | [1]DepartmentofBiology,BeijingNormalUniversity,Beijing100875,China [2]DepartmentofBiology,BeijingNormalUniv |
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| 摘 要： | Both calcium ionophore A23187 and endoplasmic reticulum Ca^2 -ATPase inhibitor thapsigargin (Tg) could increse intracellular free calcium concentration and induce apoptosis in some cell lines.In the present study,we found that HL-60 cells treated with A23187 (1μg/ml) for 4h or with Tg(0.5μg/ml) for 2h showed typical characteristics of apoptosis.Pretreatment with nontoxic concentration of cyclosporin A (CsA) (1μg/ml) could block these effects.Flow cytometric analysis of intracellular Ca^2 after staining with fluo-3 AM showed that CsA did not prevent the increase of intracellular calcium induced by A23187 or Tg,but it could maintain the high level of intracellular Ca^2 for a long time.These results suggest that CsA may prevent calcium-induced apoptosis by blocking the transportation of Ca^2 in HL-60 cells.
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| 关 键 词： | 环胞素A 钙依赖性凋亡 钙转运 HL－60细胞 抑制作用 相关性 |
Correlation between inhibition of calcium-dependent apoptosis by cyclosporin A and calcium transportation in HL-60 cells |
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| HUANG QI QING, MING FANG, HONG QING ZHANG,SHAO BAI XUE.Correlation between inhibition of calcium-dependent apoptosis by cyclosporin A and calcium transportation in HL-60 cells[J].Cell Research,1996,6(1):23-30. |
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| Authors: | HUANG QI QING MING FANG HONG QING ZHANG SHAO BAI XUE |
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| Abstract: | |
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| Keywords: | Cyclosporin A calcium ionophore A_(23187) thapsigargin apoptosis intracellular calcium |
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