NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg-protoporphyrin monomethyl ester cyclase |
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| Authors: | Stenbaek Anne Hansson Andreas Wulff Ragna Peterson Hansson Mats Dietz Karl-Josef Jensen Poul Erik |
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| Affiliation: | VKR Research Centre Pro-Active Plants, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. |
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| Abstract: | The chloroplast-localized NADPH-dependent thioredoxin reductase (NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg-protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e.g. under chloroplast biogenesis. |
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| Keywords: | ALA, 5-aminolevulinic acid Chlide, chlorophyllide H2O2, hydrogen peroxide MgP-MME, Mg-protoporphyrin monomethyl ester MgP, Mg-protoporphyrin IX NTRC, NADPH-dependent thioredoxin reductase Prx, peroxiredoxin Pchlide, protochlorophyllide PPIX, protoporphyrin IX ROS, reactive oxygen species |
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