Higher culture pH is preferable for inclusion body formation of recombinant salmon growth hormone inEsherichia
coli |
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| Authors: | Seiji Sugimoto Yoshiharu Yokoo Noritaka Hatakeyama Akira Yotsuji Sadao Teshiba Hiroshi Hagino |
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| Institution: | (1) Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahimachi, 194 Machida, Tokyo, Japan |
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| Abstract: | Summary Higher culture pH of 7.6 was shown to be preferable for the inclusion body formation of salmon growth hormone (SGH) inEscherichia
coli harboring a recombinant plasmid. High-level formation of SGH inclusion bodies was achieved at 33°C (pH 7.6). Growth inhibition by soluble SGH was also observed. |
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