| 甲壳胺诱导人肝癌HepG2细胞凋亡的实验研究 |
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| 引用本文： | 赵丽艳,孙宇,李明春,程艳芹,马守栋,曹恩惠. 甲壳胺诱导人肝癌HepG2细胞凋亡的实验研究[J]. 生物技术通讯, 2011, 22(2): 252-254. DOI: 10.3969/j.issn.1009-0002.2011.02.024 |
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| 作者姓名： | 赵丽艳 孙宇 李明春 程艳芹 马守栋 曹恩惠 |
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| 作者单位： | 1. 解放军第401医院,药剂科,山东,青岛,266071
2. 解放军第305医院,检验科,北京,100017 |
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| 基金项目： | 全军“十一五”医药卫生科研基金(06MA094) |
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| 摘 要： | 目的:通过体外实验探讨甲壳胺是否对人肝癌细胞HepG2具有生长抑制及诱导凋亡作用.方法:在HepG2细胞培养液中加入不同浓度的甲壳胺,培养48 h,于倒置相差显微镜下观察甲壳胺处理组及对照组细胞形态学变化;用流武细胞术(FCM)检测HepG2细胞的凋亡率,Western印迹检测甲壳胺处理组及对照组Bcl-2和p53蛋白...
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| 关 键 词： | 甲壳胺 细胞凋亡 人肝癌HepG2细胞 |
Apoptosis of Human Liver Cancer Cell Line HepG2 Induced by Chitosan |
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| ZHAO Li-Yan,SUN Yu,LI Ming-Chun,CHENG Yan-Qin,MA Shou-Dong,CAO En-Hui. Apoptosis of Human Liver Cancer Cell Line HepG2 Induced by Chitosan[J]. Letters in Biotechnology, 2011, 22(2): 252-254. DOI: 10.3969/j.issn.1009-0002.2011.02.024 |
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| Authors: | ZHAO Li-Yan SUN Yu LI Ming-Chun CHENG Yan-Qin MA Shou-Dong CAO En-Hui |
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| Affiliation: | ZHAO Li-Yan1,SUN Yu2,LI Ming-Chun1,CHENG Yan-Qin1,MA Shou-Dong1,CAO En-Hui1 1.Pharmacy of the PLA 401st Hospital,Qingdao 266071,2.Clinical Laboratory of the PLA 305th Hospital,Beijing 100017,China |
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| Abstract: | Objective:To study the apoptosis and proliferation inhibition in human liver cancer cell line HepG2 induced by chitosan.Methods:Inverted phase contrast microscope was used to observe the morphological changes of HepG2 induced by different concentrations of chitosan.The rate of apoptosis and the HepG2 DNA amount were examined by flow cytometry.The p53 and Bcl-2 protein expressions were assayed by Western blot.Results:Compared with the control apoptosis rate of 1.66%,the apoptosis rate of HepG2 cells increase... |
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| Keywords: | chitosan apoptosis human liver cancer cell line HepG2 |
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