| 利用TrnL-F序列探讨苎麻属植物的系统发育关系 |
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| 引用本文： | 康冬丽,贾红辉,蒋建雄.利用TrnL-F序列探讨苎麻属植物的系统发育关系[J].生物技术通讯,2011,22(1):45-48. |
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| 作者姓名： | 康冬丽 贾红辉 蒋建雄 |
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| 作者单位： | 1. 国防科学技术大学,理学院,湖南,长沙,410073
2. 湖南农业大学,细胞工程实验室,湖南,长沙,410128 |
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| 摘 要： | 目的:探讨苎麻属植物的系统发育关系。方法:对10个种和8个变种苎麻属植物的TrnL-F序列进行PCR扩增和T/A克隆产物序列测定;选取雾水葛属的植物作为参考外类群,同样也测定其TrnL-F全序列;根据TrnL-F区碱基序列的差异计算种间的遗传距离,采用UPGMA法进行系统发育分析并得到系统发育树。结果:野线麻、福州苎麻、赤麻、悬铃叶苎麻、序叶苎麻、白面苎麻、束序苎麻、密球苎麻、疏毛水苎麻、圆叶水苎麻、灰绿水苎麻和糙叶水苎麻聚成一支;帚序苎麻、黔桂苎麻、苎麻、微绿苎麻、贴毛苎麻和青叶苎麻聚为另一支;雾水葛单独聚成一类,与苎麻属的其他种在一级分支中即分开,与其他种的亲缘关系较远。结论:帚序苎麻组和苎麻组植物具有较近的亲缘关系。
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| 关 键 词： | 苎麻属 TrnL-F序列 信息位点 系统发育 |
Study on Phylogenetic Relationships of Boehmeria Based on TrnL-F Regions |
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| KANG Dong-Li,JIA Hong-Hui,JIANG Jian-Xiong.Study on Phylogenetic Relationships of Boehmeria Based on TrnL-F Regions[J].Letters in Biotechnology,2011,22(1):45-48. |
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| Authors: | KANG Dong-Li JIA Hong-Hui JIANG Jian-Xiong |
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| Institution: | KANG Dong-Li1,JIA Hong-Hui1,JIANG Jian-Xiong2 1.National University of Defense Technology,Changsha 410073,2.Cell Engineer Lab,Hunan Agriculture University,Changsha 410128,China |
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| Abstract: | Objective:To study the phylogenetic relationship of Boehmeria.Methods:we amplied the sequences of TrnL-F from ten species and eight varieties of Boehmeria by PCR amplification and directly sequencing.By referring to the Pouzolzia zeylanica,its sequence of TrnL-F was set to determined too.Based on the difference in TrnL-F,the genetic distance among varieties were calculated and UPGMA tree was constructed to analyze the genetic relationships of Boehmeria.Results:The phylogenetic trees based on the data set sh... |
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| Keywords: | Boehmeria TrnL-F information sites phylogenetic relationship |
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