| 重组人骨保护素片段OPG179与人血清白蛋白在毕赤酵母中的融合表达 |
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| 引用本文： | 刘俊丽,刘波,宋淑军,司少艳,谭小青,吴军,景青萍,张建中. 重组人骨保护素片段OPG179与人血清白蛋白在毕赤酵母中的融合表达[J]. 生物技术通讯, 2011, 22(1): 15-18,23. DOI: 10.3969/j.issn.1009-0002.2011.01.004 |
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| 作者姓名： | 刘俊丽 刘波 宋淑军 司少艳 谭小青 吴军 景青萍 张建中 |
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| 作者单位： | 1. 解放军第306医院,北京,100101
2. 军事医学科学院生物工程研究所,北京,100071 |
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| 基金项目： | 总装后司令部及卫生局项目(07ZH10); 306医院院级课题(07QN10) |
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| 摘 要： | 目的:在毕赤酵母中融合表达人骨保护素(OPG)片段OPG179与人血清白蛋白(HSA).方法:通过RT-PCR扩增获得OPG179基因,构建表达质粒pHILD2-rhOPG179-HSA,转化至毕赤酵母菌中进行表达、纯化,对分泌表达产物进行SDS-PAGE及Western印迹检测.结果:酶切鉴定与测序结果显示重组表达质...
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| 关 键 词： | 人骨保护素 人血清白蛋白 融合蛋白 毕赤酵母 表达 纯化 |
Expression of the rhOPG179-HSA Fusion Protein in Pichia pastoris |
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| LIU Jun-Li,LIU Bo,SONG Shu-Jun,SI Shao-Yan,TAN Xiao-Qing,WU Jun,JING Qing-Ping,ZHANG Jian-Zhong. Expression of the rhOPG179-HSA Fusion Protein in Pichia pastoris[J]. Letters in Biotechnology, 2011, 22(1): 15-18,23. DOI: 10.3969/j.issn.1009-0002.2011.01.004 |
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| Authors: | LIU Jun-Li LIU Bo SONG Shu-Jun SI Shao-Yan TAN Xiao-Qing WU Jun JING Qing-Ping ZHANG Jian-Zhong |
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| Affiliation: | LIU Jun-Li1,LIU Bo2,SONG Shu-Jun1,SI Shao-Yan1,TAN Xiao-Qing1,WU Jun2,JING Qing-Ping1,ZHANG Jian-Zhong1 1.The 306th Hospital of PLA,Beijing 100101,2.Beijing Institute of Biotechnology,Beiijing 100071,China |
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| Abstract: | Objective:To express in Pichia pastoris and purify recombinant human osteoprogerin(OPG) fragment OPG179 and human serum albumin(HSA) fusion protein.Methods:The gene of OPG179 was amplified by RT-PCR.The plasmid of pHILD2-rhOPG179-HSA was constructed and then transformed to Pichia pastoris.The rhOPG179-HSA fusion protein was expressed,purified,and analyzed with SDS-PAGE and Western blot.Results:The expression plasmid of pHILD2-rhOPG179-HSA was proved correct after the plasmid was identified and sequenced.The... |
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| Keywords: | recombinant human osteoprogerin human serum albumin fusion protein Pichia pastoris expression purification |
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