Expression of a New Cold Shock Protein of 21.5 kDa and of the Major Cold Shock Protein by Streptococcus thermophilus After Cold Shock |
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Authors: | Clarisse Perrin Christiane Guimont Patrice Bracquart Jean-Luc Gaillard |
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Institution: | (1) Laboratoire des BioSciences de l'Aliment, Unité Associée à l'INRA (département TPA) Université Henri Poincaré-Nancy I, Faculté des Sciences, Domaine Victor Grignard, B. P. 239, 54506 Vandoeuvre-lès-Nancy, France , FR |
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Abstract: | Streptococcus thermophilus is widely used in food fermentations; it commonly suffers diverse stress challenges during manufacturing. This study investigated
the cold shock response of S. thermophilus when the cell culture temperature shifted from 42°C to 15°C or 20°C. The growth of cells was affected more drastically after
cold shock at 15°C than at 20°C. The generation time was increased by a factor of 19 when the temperature was lowered from
42° to 20°C, and by a factor of 72 after a cold shock at 15°C. The two-dimensional electrophoretic protein patterns of S. thermophilus under cold shock conditions were compared with the reference protein pattern when cells were grown at optimal temperature.
Two proteins of 21.5 and 7.5 kDa synthesized in response to cold shock were characterized. N-terminal sequencing and sequence
homology searches have shown that the 7.5-kDa protein belonged to the family of the major cold shock proteins, while no homology
was found for the new cold shock protein of 21.5 kDa.
Received: 4 June 1999 / Accepted: 6 July 1999 |
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