The response ofRhizobium meliloti to L-methionine DL-sulphoximine |
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| Authors: | S Prakash Rao Usha George V V Modi |
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| Institution: | (1) Department of Microbiology, Faculty of Science, M.S. University of Baroda, 390 002 Baroda, India;(2) Present address: Department of Biology, McGill University, Montreal, Canada |
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| Abstract: | A strain ofRhizobium meliloti has been shown to be capable of growth in the presence of methionine sulphoximine concentrations at least two orders of magnitude
higher than that required for the complete inhibition of glutamine synthetase activity. Neither the specific growth rate,
nor the nutritional requirements of the organism were affected by methionine sulphoximine in the medium.Rhizobium meliloti appeared to assimilate ammoniavia the glutamate dehydrogenase pathway during growth in the presence of methionine sulphoximine. This suggests thatRhizobium meliloti may have some regulatory mechanism controlling ammonia assimilation that is not present in other enterobacteria possessing
similar enzymatic machinery |
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| Keywords: | Rhizobium meliloti methionine sulphoximine glutamine synthetase glutamate dehydrogenase |
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