Selective replenishment of two vesicle pools depends on the source of Ca2+ at the Drosophila synapse |
| |
Authors: | Kuromi Hiroshi Kidokoro Yoshiaki |
| |
Institution: | Institute for Behavioral Sciences, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi, Japan. kuromi@med.gunma-u.ac.jp |
| |
Abstract: | After synaptic vesicles (SVs) undergo exocytosis, SV pools are replenished by recycling SVs at nerve terminals. At Drosophila neuromuscular synapses, there are two distinct SV pools (i.e., the exo/endo cycling pool (ECP), which primarily maintains synaptic transmission, and the reserve pool (RP), which participates in synaptic transmission only during tetanic stimulation). Labeling endocytosed vesicular structures with a fluorescent styryl dye, FM1-43, and measuring intracellular Ca2+ concentrations with a Ca2+ indicator, rhod-2, we show here that the ECP is replenished by SVs endocytosed during stimulation, and this process depends on external Ca2+. In contrast, the RP is refilled after cessation of tetanus by a process mediated by Ca2+ released from internal stores. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|