Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention |
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Authors: | Hans G Drexler Cord C Uphoff |
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Institution: | (1) Department of Human and Animal Cell Cultures, DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany (Author for correspondence; E-mail |
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Abstract: | The contamination of cell cultures by mycoplasmas remains a major problem in cell culture. Mycoplasmas can produce a virtually
unlimited variety of effects in the cultures they infect. These organisms are resistant to most antibiotics commonly employed
in cell cultures. Here we provide a concise overview of the current knowledge on: (1) the incidence and sources of mycoplasma
contamination in cell cultures, the mycoplasma species most commonly detected in cell cultures, and the effects of mycoplasmas
on the function and activities of infected cell cultures; (2) the various techniques available for the detection of mycoplasmas
with particular emphasis on the most reliable detection methods; (3) the various methods available for the elimination of
mycoplasmas highlighting antibiotic treatment; and (4) the recommended procedures and working protocols for the detection,
elimination and prevention of mycoplasma contamination. The availability of accurate, sensitive and reliable detection methods
and the application of robust and successful elimination methods provide powerful means for overcoming the problem of mycoplasma
contamination in cell cultures.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | antibiotics cell lines contamination detection elimination incidence mycoplasma prevention |
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