Molecular mapping of soybean aphid resistance genes in PI 567541B |
| |
Authors: | Guorong Zhang Cuihua Gu Dechun Wang |
| |
Institution: | (1) Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824, USA |
| |
Abstract: | The soybean aphid (Aphis glycines Matsumura) is an important pest of soybean Glycine max (L.) Merr.] in North America since it was first reported in 2000. PI 567541B is a newly discovered aphid resistance germplasm
with early maturity characteristics. The objectives of this study were to map and validate the aphid resistance genes in PI
567541B using molecular markers. A mapping population of 228 F3 derived lines was investigated for the aphid resistance in both field and greenhouse trials. Two quantitative trait loci
(QTLs) controlling the aphid resistance were found using the composite interval mapping method. These two QTLs were localized
on linkage groups (LGs) F and M. PI 567541B conferred resistant alleles at both loci. An additive × additive interaction between
these two QTLs was identified using the multiple interval mapping method. These two QTLs combined with their interaction explained
most of the phenotypic variation in both field and greenhouse trials. In general, the QTL on LG F had less effect than the
one on LG M, especially in the greenhouse trial. These two QTLs were further validated using an independent population. The
effects of these two QTLs were also confirmed using 50 advanced breeding lines, which were all derived from PI 567541B and
had various genetic backgrounds. Hence, these two QTLs identified and validated in this study could be useful in improving
soybean aphid resistance by marker-assisted selection. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|