Optimal conditions for breaking medically important yeasts by an inexpensive and simple method |
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Authors: | K C Hazen J E Cutler |
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Institution: | (1) Department of Microbiology, Montana State University, 59717 Bozeman, MT, USA |
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Abstract: | Conditions for breaking various medically important yeasts using glass beads, 30 ml Corex centrifuge tubes, and a Vortex mixer were determined. From 75–95% ofCandida hyphal cells and all species of yeasts exceptSporothrix schenckii were broken when 10 g of 0.45–0.50 mm glass beads, 50–300 mg of wet cells in 5 ml of buffer, and 90 s of vortexing were employed. Yeasts ofSporothrix schenckii broke more efficiently when 0.25–0.30 mm beads were used. |
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