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The fungus-specificity of mycorrhization helper bacteria (MHBs) used as an alternative to soil fumigation for ectomycorrhizal inoculation of bare-root Douglas-fir planting stocks with Laccaria laccata
Authors:R. Duponnois  J. Garbaye  D. Bouchard  J. L. Churin
Affiliation:(1) Centre de Recherches Forestières de Nancy, F-54280 Champenoux, France
Abstract:Mycorrhization helper bacteria (MHBs) isolated and selected from the Douglas fir-Laccaria laccata symbiotic system have previously been shown to be fungus-specific: they promote ectomycorrhizal establishment of Laccaria laccata but inhibit mycorrhiza formation by other fungi. In this paper, two experiments in a nursery producing two years-old bare-root Douglas-fir planting stocks confirm the specificity of MHBs under field conditions. They also show that, by selectively helping the introduced L. laccata against the resident symbionts, MHBs are an interesting alternative (safer and easier) to soil fumigation for the success of routine controlled mycorrhization of planting stocks in forest nurseries.
Keywords:bacteria  ectomycorrhizas  Hebeloma cylindrosporum  Laccaria bicolor  Laccaria laccata  nursery  Paxillus involutus  planting stocks  Pseudomonas fluorescens  Pseudotsuga menziesii  soil fumigation
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