| 重组毕赤酵母产青霉素G酰化酶的分批发酵动力学研究 |
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| 引用本文： | 谭云,黎继烈,王卫,罗倩,朱晓媛.重组毕赤酵母产青霉素G酰化酶的分批发酵动力学研究[J].菌物学报,2016,35(1):94-103. |
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| 作者姓名： | 谭云 黎继烈 王卫 罗倩 朱晓媛 |
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| 作者单位： | 中南林业科技大学 经济林培育与利用湖南省2011协同创新中心 经济林培育与保护省部共建教育部重点实验室 湖南 长沙 410004 |
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| 基金项目： | 生物学湖南省十二五重点建设学科，中南林业科技大学研究生科技创新基金 |
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| 摘 要： | 构建了重组毕赤酵母产青霉素G酰化酶的分批发酵动力学模型。实验考察了分批发酵过程中甘油消耗、甲醇浓度、菌体浓度、溶氧、补料时间对青霉素G酰化酶活力的影响。应用Matlab软件,对菌体生长、基质消耗和产物生成方程进行最优参数估算和非线性拟合,得到相应的动力学模型。模型的计算值与实验值能较好地拟合,表明所建模型能较好反映重组毕赤酵母产青霉素G酰化酶的分批发酵过程。
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| 关 键 词： | 重组毕赤酵母 青霉素G酰化酶 发酵动力学模型 |
| 收稿时间： | 2014-06-17 |
A kinetic study of batch fermentation for penicillin G acylase enzyme production by recombinant Pichia pastoris |
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| Authors: | Yun TAN Ji-Lie LI Wei WANG Qian LUO Xiao-Yuan ZHU |
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| Institution: | Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan ProvinceKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry & Technology, Changsha, Hunan 410004, China |
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| Abstract: | A kinetic model of batch fermentation for penicillin G acylase (PGA) enzyme production by recombinant Pichia pastoris was established. The influences of glycerol consumption, methanol concentration, Pichia pastoris concentration, dissolved oxygen and feeding time on the PGA activity were investigated in batch fermentation process. Using matlab software, the kinetics model for cell growth with multisubstrates, PGA production and substrate consumption was established through optimal parameter estimation and nonlinear fitting. The model calculation fitted well with the experimental observations, and showed that the constructed kinetic models could well reflect the batch fermentation for PGA production by recombinant P. pastoris. |
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| Keywords: | recombinant Pichia pastoris penicillin G acylation enzyme kinetic models of fermentation |
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