大鼠肾硫酸基转移酶基因的cDNA克隆及序列分析

在许多激素、神经递质、药物和异生化合物的生物转化中，硫酸酯化反应是一重要的代谢途径［１，２］．这些化合物的硫酸酯化通常导致其生物活性的降低及尿排泄量的增加．硫酸酯化是把３′－磷酸腺苷－５′磷酸硫酸（ＰＡＰＳ）的活性硫酸根转移到某一底物（如Ｒ－ＯＨ）．...

The cDNA Cloning and Sequencing of a Sulfotransferase from Rat Kindey

Sulfotransferase (ST) enzymes catalyze the sulfate conjugation of many hormones,neurotransmitters,drugs and xenobiotic compounds.In order to study molecular biology of rat kidney ST,cDNA library of rat kidney in pcDNA1vector was synthesized by Gubler's method.Then,the library was screened with labled oligonucleotide as probe,and consequently,four positive clones were selected as possible sulfotransferase.One of them was sequenced by the dideoxyl chain termination method.It showed that the full length cDNA fragment(namely STK 63 )coutained 1277 bp and deduced amino acid of 296,which shared 84% amino acid identity with a human liver sulfotransferase in the GenBank TM/EMBL Data Bank with acceession number U66036 prot.Northern blot anlysis was performed with RNA samples of kidney,heart,liver,muscle,stomach,lung,intestine,spleen,testis and brain.A band hybridized with STK 63 cDNA fragment was detected at about 1.3 kilobase in kidney RNA sample,but no hybridization band was observed in other tissues.It implied that STK 63 cloning might be a specfic rat kidney sulfotransferase.