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The human genome contains multiple sequences of varying homology to mouse mammary tumour virus DNA |
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| Authors: | B Westley F E May |
| Institution: | Unité d''Endocrinologie Cellulaire et Moléculaire, U 148 INSERM, 60 rue de Navacelles, 34100 Montpellier France Tel. (67) 54.15.44 |
| Abstract: | Sequences related to the mouse mammary tumour virus (MuMTV) DNA were isolated from a genomic library of human DNA by screening under conditions of relaxed stringency. It is estimated that there are in the order of 50 MuMTV-like sequences per haploid genome and that the homology between the different human sequences and MuMTV varies by 15%. |
| Keywords: | Recombinant DNA retroviruses gene library phage lambda vector nucleic acid hybridisation bp base pairs EtBr ethidium bromide kb 1000 bp MoMLV Moloney murine leukaemia virus MuMTV mouse mammary tumour virus pfu plaque-forming units SDS sodium dodecyl sulfate SSC 150 mM NaCl 15 mM sodium citrate pH 7 6 |
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