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Pumpkin (Cucurbita sp.) seed globulin III. Comparison of subunit structures among seed globulins of various Cucurbita species and characterization of peptide components
Authors:Hara  Ikuko; Ohmiya  Morikazu; Matsubara  Hiroshi
Institution:Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka 560, Japan
Abstract:Various Cucurbita seed globulins showed patterns similar toone another on SDS-gel electrophoresis, {alpha} and ß bandsfor unreduced globulins and {gamma}, {gamma}', {delta} and {delta}' bands for reduced ones.On gel electrophoresis in 6 M urea, reduced globulin gave twoacidic and two basic bands. These corresponded to {gamma} and {gamma}' chainsand {delta}1 and {delta}2 chains, respectively, identified by two-dimensionalurea-SDS gel electrophoresis. The compositions of the {alpha} and ßsubunits were proposed. (Received September 8, 1977; )
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