High-resolution mapping by YAC fragmentation of a 2.5-Mb Xp22 region containing the human RS, KFSD and CLS disease genes |
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Authors: | Esther Van de Vosse Paola Van der Bent Joris J Heus Gert- Jan B Van Ommen Johan T Den Dunnen |
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Institution: | (1) MGC-Department of Human Genetics, Leiden University, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands, NL |
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Abstract: | The disease loci for X-linked Retinoschisis (RS), Keratosis follicularis spinulosa decalvans (KFSD), and Coffin-Lowry syndrome
(CLS) have been localized to the same, small region in Xp22 on the human X Chromosome (Chr). To generate a high-resolution
map of the available contig in this area, we have used the YAC fragmentation vectors pBP108/ADE2 and pBP109/ADE2 and generated fragmented YACs from a 2.5-Mb YAC (y939H7) spanning the mentioned disease gene candidate regions. Forty-seven
fragmented YACs were generated and analyzed, ranging in size from 170 kb to over 2400 kb. The resulting YAC fragmentation
panel was used to construct a detailed restriction map of the region and has been used to bin clones and markers. As a deletion
panel, it will present a valuable resource for further mapping.
Received: 31 December 1996 / Accepted: 22 February 1997 |
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