Covalently bound flavin as prosthetic group of choline oxidase. |
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| Authors: | N Ohishi K Yagi |
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| Affiliation: | Institute of Biochemistry, Faculty of Medicine, University of Nagoya, Nagoya 466, Japan |
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| Abstract: | Highly purified choline oxidase of fluoresced as a yellow band on SDS gel in 7% acetic acid. The absorption spectrum of the enzyme showed marked hypsochromic shift of the second absorption band. Aminoacyl flavin obtained from this enzyme was identified with 8α-[N(3)-histidyl]FAD. |
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