Somatic embryo formation and germination from immature embryo-derived suspension-cultured cells of Angelica sinensis (Oliv.) Diels |
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Authors: | H S Tsay H L Huang |
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Institution: | (1) Department of Agronomy, Taiwan Agricultural Research Institute, Wufeng, Taichung 41301, Taiwan Fax no.: +886-4-3338162, TW |
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Abstract: | Embryogenic callus was induced from immature embryos of Angelica sinensis cultured on Murashige and Skoog (MS) basal medium. Embryogenic callus growth was more rapid on MS basal medium than on B5
or White medium. Embryogenic callus was used to establish a suspension culture and somatic embryos and germinating embryos
developed during the culture. A shaking speed of 80 rpm was found to be optimal for establishing suspension cultures, while
100 rpm produced more somatic embryos and germinating embryos with an initiation cell density of 0.2 ml packed cell volume/25
ml medium. Adding 0.3% agar to the liquid medium also stimulated the formation of somatic and germinating embryos. While no
plant growth regulators were needed for culture initiation and plant regeneration, the addition of 0.5–1 mg/l 2,4-dichlorophenoxyacetic
acid was needed to maintain the embryogenic suspension culture by preventing embryo germination. Forty percent of the germinating
embryos survived after culturing on filter paper moistened with liquid half-strength MS medium containing 3% sucrose. The
plants were successfully transferred into soil.
Received: 19 March 1997 / Revision received: 21 November 1997 / Accepted: 19 January 1998 |
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Keywords: | Angelica sinensis Immature embryo culture Somatic embryo Suspension cell culture |
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