Quantitative characterization of domes in primary mouse mammary epithelial tumor cell cultures |
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Authors: | Young Lawrence J T Cardiff Robert D Seeley Thomas |
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Institution: | (1) Department of Pathology, University of California, School of Medicine, Davis, California |
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Abstract: | Summary Dome populations from primary cultures of mouse mammary tumor cells were quantitatively studied in regard to size, distribution,
density and the area occupied by light-diffusion photography and image analysis. The effects of fetal bovine serum, insulin
and hydrocortisone were analyzed. Quantitative characterization documented dome diameter (mode diameter 0.26 to 0.52 mm),
dome area occupied (average 23%, maximum 38.7%), and density (average 78 domes per cm2, maximum 117 domes per cm2) for standard culture conditions. Insulin and hydrocortisone had a primary effect on dome density whereas 15% fetal bovine
serum had a minimal effect. However, insulin and hydrocortisone had a synergistic optimal effect on dome population. Time-lapse
cinematography revealed that the dome population is not static, but a very dynamic one. Domes underwent irregular pulsations
of expansion and contraction. Dome enlargement was either by a series of expansions and contractions, by lateral involvement
of other cells, or by coalescence of two or more domes. Domes have been considered to be the in vitro counterpart of the in
vivo acinus of the mouse mammary gland. However, quantitative dome population characterization has not been available. Dome
analysis by light-diffraction photography and image analysis lends itself towards correlative studies of domes and their differentiative
products.
Supported in part by Public Health Service Contract NO1-CP 61013 within the Virus Cancer Program of the National Cancer Institute
and by Public Health Service Training Grant CA05245 from the National Cancer Institute. |
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Keywords: | domes light-diffraction photography image analysis hormone synergism |
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