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Bioassay for the rapid detection of bacteriocins in fermentation broth |
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| Authors: | T. Mugochi M.P. Nandakumar R. Zvauya B. Mattiasson |
| Affiliation: | (1) Department of Biotechnology, Center for Chemistry & Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden;(2) Present address: Department of Chemical and Biochemical Engineering, UMBS, Hilltop Center 1000, Baltimore, MD 212 50, USA;(3) Department of Biochemistry, University of Zimbabwe, P.O. Box MP167, Mount Pleasant, Harare, Zimbabwe |
| Abstract: | A bioassay for the rapid detection of bacteriocins by detecting efflux of K+ ions from a bacteriocin-sensitive indicator strain was developed. There was a rapid increase in concentration of K+ in the medium from approximately 1.6 to 9.5 mM when the crude bacteriocin was added to the sensitive strain. A bacteriocin activity of 8.25 AU ml–1 produced the lowest detectable concentration of K+ (0.179 mM) and the smallest inhibition zone (0.4 mm). The smallest cell dry mass of the indicator strain capable of releasing a detectable level of K+ (0.102 mM) was 0.76 mg. Detection of the bacteriocin by measuring the efflux of K+ compares well with the conventional agar well diffusion assay. Low activities of bacteriocin and low amounts of the sensitive indicator strain biomass can be used with this bioassay. |
| Keywords: | bacteriocin bioassay bacteriocin sensitive strain potassium ion efflux |
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