Effect of lignocellulosic inhibitory compounds on growth and ethanol fermentation of newly-isolated thermotolerant Issatchenkia orientalis |
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Authors: | Kwon Yong-Jin Ma An-Zhou Li Qian Wang Feng Zhuang Guo-Qiang Liu Chun-Zhao |
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Institution: | a National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China b Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China c Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China |
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Abstract: | A newly isolated thermotolerant ethanologenic yeast strain, Issatchenkia orientalis IPE 100, was able to produce ethanol with a theoretical yield of 85% per g of glucose at 42 °C. Ethanol production was inhibited by furfural, hydroxymethylfurfural and vanillin concentrations above 5.56 g L−1, 7.81 g L−1, and 3.17 g L−1, respectively, but the strain was able to produce ethanol from enzymatically hydrolyzed steam-exploded cornstalk with 93.8% of theoretical yield and 0.91 g L−1 h−1 of productivity at 42 °C. Therefore, I. orientalis IPE 100 is a potential candidate for commercial lignocelluloses-to-ethanol production. |
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Keywords: | Thermotolerant yeast Issatchenkia orientalis Isolation Identification Inhibition |
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