Cloning and expression of stearoyl-CoA desaturase 1 (SCD-1) in the liver of the Sichuan white goose and landes goose responding to overfeeding |
| |
Authors: | Zhi-xiong Pan Chun-chun Han Ji-wen Wang Liang Li Hui Tang Jia Lv Lizhi Lu Feng Xu |
| |
Institution: | (1) Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan, 625014, People’s Republic of China;(2) Institute of Animal Science and Veterinary Medicine, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang, 310021, People’s Republic of China; |
| |
Abstract: | The EST sequence of goose (Anser cygnoides) Stearoyl-CoA desaturase 1(SCD-1) was obtained from a subtractive cDNA library. To further investigate the role of SCD-1
in lipid metabolism in geese, 5′-RACE and 3′-RACE were carried out in this study to obtain the complete cDNA sequence of goose
SCD-1, which contained a 29-bp 5′ UTR, a 1074-bp open reading frame (ORF) encoding 357 amino acids, and a 125-bp 3′ UTR. The
expression of SCD-1 was measured in several tissues, and the effects of overfeeding on the expression of SCD-1 were studied.
The results of real time RT-PCR demonstrated that, compared to the brain, goose SCD-1 mRNA was more abundant in the liver.
Overfeeding markedly increased the mRNA expression of SCD-1 in the liver of Sichuan White and Landes geese, and gene expression
was markedly higher in the Sichuan White goose than in the landes goose. The mRNA abundance of SCD-1 in the liver had significant
positive correlations with triacylglycerol (TG) content in liver lipids and in the levels of plasma insulin and very low-density
lipoproteins (VLDL) levels in Sichuan white geese. However, the mRNA abundance of SCD-1 in the livers of Landes geese had
only significant positive correlations with the TG content in liver lipids. In conclusion, SCD-1 is not only critical for
hepatic steatosis in geese but is also important for the difference in lipid deposition in the livers of the two breeds. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|