Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu |
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| Authors: | H C Wang J T Chen W C Chang |
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| Institution: | (1) Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115, Taiwan, Republic of China;(2) Institute of Biotechnology, National University of Kaohsiung, Kaohsiung, 811, Taiwan, Republic of China;(3) Department of Life Sciences, National University of Kaohsiung, Kaohsiung, 811, Taiwan, Republic of China |
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| Abstract: | Plant regeneration through somatic embryogenesis of Areca catechu L. was established using leaf, root and stem segments as explants. Embryogenic callus was induced and maintained on medium
supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro-2-methoxybenzoic acid (dicamba) at concentrations
2, 4, 6 and 8 mg dm−3 in darkness. Somatic embryos were found on primary callus in the presence of 2 and 4 mg dm−3 dicamba and during subculture on 2 – 8 mg dm−3 2,4-D or 2 – 4 mg dm−3 dicamba-containing media. Plantlet conversion from embryos was successfully achieved on growth regulator-free medium. The
plants grew well when transplanted to containers in shaded greenhouse. |
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| Keywords: | betel nut dicamba 2 4-dichlorophenoxyacetic acid |
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