| 两种树脂固相合成机体保护肽的比较 |
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| 引用本文： | 徐婉如,张俊君,陈五岭.两种树脂固相合成机体保护肽的比较[J].现代生物医学进展,2008,8(9):1674-1676,1667. |
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| 作者姓名： | 徐婉如 张俊君 陈五岭 |
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| 作者单位： | 西北大学生命科学学院,陕西西安,710069 |
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| 摘 要： | 目的:比较Wang树脂和二氯三苯甲基树脂(以下简称二氯树脂)在固相合成机体保护肽中的表现,为工业化生产提供理论依据。方法:通用的Fmoc固相合成法制备机体保护肽,通过比色法对两种树脂与氨基酸的偶联率进行比较,用质谱(MALDI-TOFMS)对粗肽进行鉴定分析,用反相高效液相色谱(RP-HPLC)进行粗肽的分析和纯化。结果:显示以二氯树脂作载体,第一个氨基酸的连接率,以及机体保护肽的纯度和产率都要明显高于Wang树脂。结论:二氯树脂作载体合成机体保护肽更适合大规模工业化生产的要求。
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| 关 键 词： | Wang树脂 二氯三苯甲基树脂 机体保护肽 |
Comparative Study of Two Resins on Solid-phase Synthesis of Organic Assistant Peptide |
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| XU Wan-ru,ZHANG Jun-jun,CHEN Wu-ling.Comparative Study of Two Resins on Solid-phase Synthesis of Organic Assistant Peptide[J].Progress in Modern Biomedicine,2008,8(9):1674-1676,1667. |
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| Authors: | XU Wan-ru ZHANG Jun-jun CHEN Wu-ling |
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| Abstract: | Objective:To compare the Wang resin and 2-chlorotrity choloride resin on solid-phase synthesis of Organic Assistant Peptide, and to provide theoretical basis for industrial manufacture.Methods:Organic Assistant Peptide was synthesized by the conven-tional solid-phase synthesis, and comparing the attaching efficiency with different resins by colorimetry.The crude production was identi-fied, analyzed and separated by MALDI-TOF MS and RP-HPLC.Results:when using Wang resin, the attaching efficiency and purity and production of Organic Assistant Peptide was obvious higher than 2-chlorotrity choloride resin.Conclusions:2-chlorotrity choloride resin is more suitable than Wang resin on solid-phase Synthesis of Organic Assistant Peptide, and can obtain good yield and purity. |
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| Keywords: | Wang resin 2-chlorotrityl choloride resin Organic AssistantPeptide |
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