Isolation and characterization of polymorphic microsatellite loci from RAPD product in half-smooth tongue sole (Cynoglossus semilaevis) and a test of cross-species amplification |
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| Authors: | Liu Y G Bao B L Liu L X Wang L Lin H |
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| Affiliation: | Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China, Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Shanghai 200090, China, Agriculture Ministry Key Laboratory for Aquatic Genetic Resources and Aquaculture Ecology, Shanghai Fisheries University, Shanghai 200090, China; Linyi Academy of Agricultural sciences, Linyi 276012, China. |
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| Abstract: | Eleven polymorphic microsatellite loci have been isolated and characterized from random amplified polymorphic DNA product in half-smooth tongue sole, Cynoglossus semilaevis. Twenty-one microsatellites were selected for designing microsatellite primers, of which 11 gave working primer pairs. They had between three and 12 alleles. Observed and expected heterozygosities varied from 0.53 to 0.93, and from 0.52 to 0.80, respectively. Five additional fish species assessed for cross-species amplification revealed between one and three positive amplifications and between zero and three polymorphic loci per species. |
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| Keywords: | cross‐species amplification Cynoglossus semilaevis half‐smooth tongue sole microsatellite RAPD |
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