PERMANENT GENETIC RESOURCES: A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis |
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Authors: | Rougeron V Waleckx E Hide M DE Meeûs T Arevalo J Llanos-Cuentas A Bañuls A L |
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Institution: | Genetique et Evolution des Maladies Infectieuses, IRD/CNRS (UMR2724), Centre, IRD, 911, Avenue Agropolis BP 64501, F-34394, Montpellier Cedex 5, France, Instituto de Medicina Tropical 'Alexander von Humboldt', Lima, Peru. |
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Abstract: | Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty‐six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 ± 4.1 and the observed heterozygosity averaging 0.425 ± 0.202. The important heterozygote deficits we observed (FIS = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania. This work validates the potential use of these microsatellites for population genetics analysis. |
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Keywords: | Leishmania braziliensis microsatellites population genetics population structure |
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