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Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco) |
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| Authors: | Froelicher Y Dambier D Bassene J B Costantino G Lotfy S Didout C Beaumont V Brottier P Risterucci A M Luro F Ollitrault P |
| Affiliation: | Cirad, UPR Multiplication végétative, San Giuliano, F-20230 San Nicolao, France, Cirad, UPR Multiplication végétative, Avenue Agropolis, F-34398 Montpellier Cedex 5, France, Inra, Unité de Génétique et écophysiologie de la qualité des agrumes, San Giuliano, F-20230 San Nicolao, France, Inra, Station d'El Menzeh, BP 293 Kénitra, Morocco, Centre de Recherche Pernod-Ricard, 120 Avenue du Maréchal Foch, F-94015 Créteil Cedex, France, Genoscope, 2 rue Gaston Cremieux, F-91057 Evry, France, Cirad, UMR 1096, Avenue Agropolis, BP 5035, F-34032 Montpellier Cedex 5, France. |
| Abstract: | A dinucleotide-enriched genomic library was obtained from mandarin orange (Citrus reticulata Blanco). A subset of 101 positive clones was sequenced and primers were designed. The loci were screened for levels of variation using 26-29 wild mandarin oranges collected in Vietnam. Forty-three loci were polymorphic with the number of alleles ranging from two to 18. The observed heterozygosity (H(O) ) and expected heterozygosity (H(E) ) were from 0.03 to 0.96 and from 0.03 to 0.92, respectively. |
| Keywords: | Citrus genetic diversity microsatellite molecular marker Rutaceae STMS |
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