Biodegradation of tributyl phosphate by novel bacteria isolated from enrichment cultures |
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Authors: | Kedar C Ahire Balu P Kapadnis Girish J Kulkarni Yogesh S Shouche Rajendra L Deopurkar |
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Institution: | (1) Department of Microbiology, University of Pune, Pune, 411 007, India;(2) Molecular Biology Unit, National Centre for Cell Science, Pune, 411007, India |
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Abstract: | Tributyl phosphate (TBP) is an organophosphorous compound, used extensively (3000–5000 tonnes/annum) as a solvent for nuclear
fuel processing and as a base stock in the formulation of fire-resistant aircraft hydraulic fluids and other applications.
Because of its wide applications and relative stability in the natural environment TBP poses the problem of pollution and
health hazards. In the present study, fifteen potent bacterial strains capable of using tributyl phosphate (TBP) as sole carbon
and phosphorus source were isolated from enrichment cultures. These isolates were identified on the basis of biochemical and
morphological characteristics and 16S rRNA gene sequence analysis. Phylogenetic analysis of 16S rRNA gene sequences revealed
that two isolates belonged to class Bacilli and thirteen to β and γ-Proteobacteria. All these isolates were found to be members
of genera Alcaligenes, Providencia, Delftia, Ralstonia, and Bacillus. These isolates were able to tolerate and degrade up to 5 mM TBP, the highest concentration reported to date. The GC–MS method
was developed to monitor TBP degradation. Two strains, Providencia sp. BGW4 and Delftia sp. BGW1 showed respectively, 61.0 ± 2.8% and 57.0 ± 2.0% TBP degradation within 4 days. The degradation rate constants,
calculated by first order kinetic model were between 0.0024 and 0.0099 h−1. These bacterial strains are novel for TBP degradation and could be used as an important bioresource for efficient decontamination
of TBP polluted waste streams. |
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