Enhancement of anthraquinone production in Morinda citrifolia cell suspension cultures after stimulation of the proline cycle with two proline analogs |
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Authors: | Quevedo Carla V Perassolo María Giulietti Ana M Rodríguez Talou Julián |
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Institution: | (1) C?tedra de Microbiolog?a Industrial y Biotecnolog?a, Facultad de Farmacia y Bioqu?mica, Universidad de Buenos Aires, Jun?n 956, 1113, Ciudad Aut?noma de Buenos Aires, Argentina; |
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Abstract: | Synthesis of anthraquinones (AQs) involves the shikimate and 2-C-methyl-D-erythritol 4-phosphate pathways. The proline cycle is linked to the pentose phosphate pathway (PPP) to generate
NADPH needed in the first steps of this pathway. The effect of two proline analogs, azetidine-2-carboxylic acid (A2C) and
thiazolidine-4-carboxylic acid (T4C), were evaluated in Morinda citrifolia suspension cultures. Both analogs gave higher proline accumulation after 6 and 10 days (68 and 179% after 6 days with A2C
at 25 and 50 μM, respectively, and 111% with T4C added at 100 μM). Induction of the proline cycle increased the AQ content
after 6 days (~40% for 50 μM A2C and 100 μM T4C). Whereas A2C (50 μM) increased only AQ production, T4C also enhanced total
phenolics. However, no induction of the PPP was observed with any of the treatments. This pathway therefore does not limit
the supply of carbon skeletons to secondary metabolic pathways. |
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