Characterization of Thinopyrum bessarabicum chromosome segments in wheat using random amplified polymorphic DNAs (RAPDs) and genomic in situ hybridization期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Characterization of Thinopyrum bessarabicum chromosome segments in wheat using random amplified polymorphic DNAs (RAPDs) and genomic in situ hybridization

Authors:	I. P. King K. A. Purdie H. N. Rezanoor R. M. D. Koebner T. E. Miller S. M. Reader P. Nicholson

Affiliation:	(1) Cambridge Laboratory, IPSR, JI Centre, NR4 7UJ Colney, Norwich, Norfolk, UK

Abstract:	Ten random amplified polymorphic DNA (RAPD) markers specific to chromosome 5E^b of Thinopyrum bessarabicum were detected. Genomic in situ hybridization and standard cytological observations revealed that six of the markers are located on the 5E^b short arm and four are located on the 5E^b long arm. These RAPD markers have been used to confirm the identity of putative 5E^b (5A) and 5E^b (5D) substitution individuals. The potential of RAPDs for the detection of wheat/alien recombinants is discussed.

Keywords:	Random amplified polymorphic DNA Genomic in situ hybridization Wheat Thinopyrum bessarabicum Isozymes
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