| 丹参SmGGPPS3基因的克隆及表达分析 |
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| 引用本文： | 化文平,宋双红,智媛,王喆之. 丹参SmGGPPS3基因的克隆及表达分析[J]. 武汉植物学研究, 2014, 0(1): 50-57 |
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| 作者姓名： | 化文平 宋双红 智媛 王喆之 |
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| 作者单位： | [1]药用资源与天然药物化学教育部重点实验室,西北濒危药材资源开发国家工程实验室,陕西师范大学,西安710062 [2]陕西学前师范学院生物科学与技术系,西安710062 |
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| 基金项目： | 陕西省教育厅项目（12JK0833）;陕西省博士后科研项目;陕西学前师范学院基金（11KJ007）. |
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| 摘 要： | 香叶基香叶基焦磷酸合酶(Geranylgeranyl pyrophosphate synthase,GGPPS)是植物细胞二萜类物质合成的重要调节靶点。本研究从药用植物丹参中克隆了一条新的GGPPS基因(SmGGPPS3),基因全长2908 bp,包含一个931 bp的内含子和一个960 bp的编码序列。推测的氨基酸序列与蓖麻、橡胶、拟南芥等植物GGPPS一致性达到67%以上。实时定量PCR结果显示,SmGGPPS3基因在丹参不同发育时期不同器官中表达差异显著,同时受茉莉酸甲酯和病原菌的诱导。遗传互补实验也表明,SmGGPPS3编码蛋白具有GGPP合酶的活性。
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| 关 键 词： | 丹参 香叶基香叶基焦磷酸合酶(GGPPS) 基因克隆 基因表达 |
Cloning and Expression Analysis of the Gene from Salvia miltiorrhiza |
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| Affiliation: | HUA Wen-Ping, SONG Shuang-Hong , ZHI Yuan , WANG Zhe-Zhi ( 1. Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi＇an, 710062, China; 2. Department of Life Sciences and Technology, Shaanxi Xueqian Normal University, Xi＇an 710100, China) |
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| Abstract: | Geranylgeranyl pyrophosphate synthase （GGPPS） is an important target site for regulating diterpenoid biosynthesis in plant ceils. Now, a novel geranylgeranyl pyrophosphate synthase gene （SmGGPPS3） was cloned from the medicinal plant Salvia miltiorrhiza. The full- length SmGGPPS3 consists of 2908 nucleotides, including a 931-bp intron and one 960-bp ORF encoding region. The deduced protein of SmGGPPS3 showed more than 67% similarity with GGPPSs from Ricinus communis, rubber and Arabidopsis. Quantitative RT-PCR analysis revealed that SmGGPPS3 was significantly expressed in different development stages and different tissues examined, and could be induced by methyl jasmonate（MeJA） and pathogens. Furthermore, the genetic complementation expression of SmGGPPS3 in Escherichia coil revealed that SmGGPPS3 encoded a functional GGPP synthase. |
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| Keywords: | Salvia miltiorrhiza GGPPS Gene clone Gene expression |
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