| 秦艽体细胞胚不同发育阶段几种同工酶的特性 |
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| 引用本文： | 潘有福,王星,孟玉玲,王仑山,杨景宇.秦艽体细胞胚不同发育阶段几种同工酶的特性[J].西北植物学报,1995,15(5). |
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| 作者姓名： | 潘有福 王星 孟玉玲 王仑山 杨景宇 |
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| 作者单位： | 兰州大学生物系，天水师专生物系 |
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| 摘 要： | 以秦艽（ＧｅｎｔｉａｎａｃｒａｓｓｉｃａｕｌｉｓＤｕｔｈｉｅｅｘＢｕｒｋｉｌｌ）无菌苗幼嫩茎叶为外植体，在ＭＳ培养基上诱导出胚性愈伤组织及体细胞胚。本文比较了秦荒体细胞胚发育过程中几种酶的活性及同工酶变化。过氧化物酶与细胞色素氧化酶均在球形胚阶段出现一活性高峰；酯酶同工酶在体细胞胚发育过程中伴有特征带Ｅ＿２、Ｅ＿６的出现；酸性磷酸酯酶的活性与体细胞胚发育程度呈正相关。所以认为只要综合运用这几种同工酶的实验数据，就可以灵敏检测体细胞胚的发育进程。
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| 关 键 词： | 秦艽，体细胞胚，过氧化物酶，细胞色素氧化酶，酯酶，酸性磷酸酯酶 |
CHARACTERIZATION OF SEVERAL ISOZYMES FROM EMBRYOGENIC CALLI AND SOMATIC EMBRYOS AT DIFFERENT DEVELOPMENTAL STAGES OF GENTLANACRASSICAULIS DUTHIE EX BURKILL |
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| Pan Youfu,WangXing,Meng Yuling and Wang Lunshan.CHARACTERIZATION OF SEVERAL ISOZYMES FROM EMBRYOGENIC CALLI AND SOMATIC EMBRYOS AT DIFFERENT DEVELOPMENTAL STAGES OF GENTLANACRASSICAULIS DUTHIE EX BURKILL[J].Acta Botanica Boreali-Occidentalia Sinica,1995,15(5). |
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| Authors: | Pan Youfu WangXing Meng Yuling and Wang Lunshan |
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| Abstract: | Embryogenic calli and the somatic embryos at different stages were obtained when segmentsof young leaves and stems of Gentiana crassicaulis Duthie ex Burkill were cultured on MS medi-um.The activities and isozymic patterns of several isozymes from embryogenic calli and somaticembryos at different stages were analyzed.The change of peroxidase was very similar to cy-tochrome oxidase. Their activities got highest together at glohlar stage.There were two specificbaiids E_2,E_6,appeared in the isozymic patterns of esterase,The activity of acid phosphatase waspositive correlation with the degree that somatic embryos developmented to.Our analysis of theactivities and electrophoretic patterns of the several enzymes proves to be an effect approach tothe characterization of developmental procession of somatic embryos. |
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| Keywords: | Gentiana crassicaulis Duthie ex Burkill somatic embryos peroxidase cytochromeoxidase esterase acid phosphatase |
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