Molecular cloning of theEnvC gene ofEscherichia coli |
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Authors: | Dr Jürgen Robert Klein Bernhard Henrich Roland Plapp |
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Institution: | (1) Department of Biology, Division of Microbiology, University of Kaiserslautern, Kaiserslautern, Germany;(2) Fachbereich Biologie, Abteilung Mikrobiologie, Universität Kaiserslautern, D-675 Kaiserslautern, Germany |
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Abstract: | DNA fragments complementing theenvC mutation could be isolated by cloning chromosomal DNA in the vector pUH84. When the frequencies of transformation and the frequencies of restoring theenvC
+ phenotype were compared, the high copy number hybrid plasmids complemented with a frequency of 10–5. After subcloning theenvC-complementing DNA fragment into the low copy number plasmid pLG339, efficient complementation was achieved by spontaneous integration of the IS2 element ofEscherichia coli. By nucleotide sequence analysis, a potential promoter, a ribosome-binding site, and an unidentified reading frame were detected in the respective DNA fragment. |
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