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In vitro assessment of Fusarium head blight spp. on wheat cultivars
Authors:Nachaat Sakr
Affiliation:Department of Agriculture, Atomic Energy Commission of Syria, Damascus, Syria
Abstract:Aggressiveness in four isolates of Fusarium head blight (FHB) species (F. culmorum, F. solani, F. verticillioides and F. equiesti) was studied in vitro on six wheat cultivars using a modified Petri-dish test. Results showed differences between cultivars inoculated with FHB isolates and control for three aggressiveness criteria: germination rate reduction, standardised area under disease progress curve (AUDPCstandard), and coleoptile length reduction. Regarding AUDPCstandard and Petri-dish aggressiveness index, significant differences were detected among fungal isolates. The other two aggressiveness criteria: germination rate reduction and coleoptile length reduction did not distinguish between FHB isolates. The Petri-dish test was repeatable and stable method to assess aggressiveness of four FHB species for all tested wheat cultivars. The current study confirmed the suitability of in vitro modified Petri-dish method to be used as fast and reliable test to analyse aggressiveness in FHB species.
Keywords:FHB-wheat system  local wheat cultivars  Petri-dish test
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