| 草鱼诱导型一氧化氮合酶cDNA和启动子的克隆及分析 |
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| 引用本文： | 汪新艳,赵太强,郭嘉聪,杨牧,周红.草鱼诱导型一氧化氮合酶cDNA和启动子的克隆及分析[J].四川动物,2011,30(4). |
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| 作者姓名： | 汪新艳 赵太强 郭嘉聪 杨牧 周红 |
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| 作者单位： | 电子科技大学生命科学与技术学院,成都,610054 |
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| 基金项目： | 电子科技大学青年科学基金项目资助，国家自然科学基金 |
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| 摘 要： | 应用PCR和RACE技术,以细菌脂多糖(LPS)刺激的草鱼头肾白细胞cDNA为模板,获得草鱼iNOS (Inducible nitric oxide synthase)全长cDNA序列.该序列共4286 bp,编码含1080个氨基酸残基的蛋白.氨基酸序列比对分析发现草鱼iNOS与金鱼iNOS a和b、斑马鱼iNOS 2b以及鲤鱼iNOS高度保守,序列一致性均大于80%;它们的血红素、四氢生物蝶呤、钙调蛋白、FMN、FAD和NADH等辅因子结合区域也十分保守.用NJ法对新获得的序列和其它脊椎动物NOS的编码序列进行进化分析证实它属于诱导型NOS.在此基础上,运用基因步移技术分离草鱼iNOS基因的启动子序列,共有1978 bp.生物信息学分析发现该序列含有包括GR、AP1、C/EBP、ER、YY1、IRF1和IL-6 REBP在内的多个转录因子的潜在结合位点.
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| 关 键 词： | cDNA序列 启动子序列 序列分析 |
Cloning and Sequence Analysis of the cDNA and Promoter of Grass Carp Inducible Nitric Oxide Synthase |
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| WANG Xin-yan,ZHAO Tai-qiang,GUO Jia-cong,YANG Mu,ZHOU Hong.Cloning and Sequence Analysis of the cDNA and Promoter of Grass Carp Inducible Nitric Oxide Synthase[J].Sichuan Journal of Zoology,2011,30(4). |
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| Authors: | WANG Xin-yan ZHAO Tai-qiang GUO Jia-cong YANG Mu ZHOU Hong |
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| Institution: | WANG Xin-yan,ZHAO Tai-qiang,GUO Jia-cong,YANG Mu,ZHOU Hong(College of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054,China) |
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| Abstract: | The full length cDNA of grass carp iNOS was isolated from LPS-stimulated grass carp head kidney leucocytes by using PCR amplification coupling to 5'-and 3'-RACE.This cDNA was 4286 bp in length which encodes a protein with 1080 amino acid residues.Amino acid alignment showed that the identities between grass carp iNOS and goldfish iNOS,zebrafish iNOS2b and common carp iNOS were all more than 80%.The cofactor-binding sites for heme,tetrahydrobiopterin,calmodulin,FMN,FAD pyrophosphate,FAD isoalloxazine,NADPH r... |
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| Keywords: | iNOS |
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