Biochemical and Immunocytochemical Evidence for a Deficiency of Normal Interfascicular Oligodendroglia in the CNS of the Dysmyelinating Mutant (md) Rat |
| |
Authors: | Susan Kahn Francine A Tansey Wendy Cammer |
| |
Institution: | Departments of Neurology and Neuroscience, Albert Einstein College of Medicine, Bronx, New York, U.S.A. |
| |
Abstract: | Carbonic anhydrase was assayed and carbonic anhydrase and 5'-nucleotidase were localized in the CNS of myelin-deficient mutant rats and normal littermates. The carbonic anhydrase specific activities were reduced by 61% and 29% in the mutants' forebrains and cerebella, respectively, and the total carbonic anhydrase activity in the spinal cords was reduced by 35%. Immunostained cells were found in gray matter from both normal and mutant rats, but, in the mutants, there was a marked deficiency of interfascicular oligodendrocytes in the regions that are normally occupied by white matter. It is suggested that a developmental study could indicate the step(s) at which normal differentiation of interfascicular oligodendroglia is blocked in this mutant. |
| |
Keywords: | Oligodendroglia Carbonic anhydrase 5'-Nucleotidase Myelin Glial cells |
|
|