Induction of isoflavonoid and retrochalcone branches of the flavonoid pathway in cultured Glycyrrhiza echinata cells treated with yeast extract. |
| |
| Authors: | K Nakamura T Akashi T Aoki K Kawaguchi S Ayabe |
| |
| Institution: | Department of Applied Biological Sciences, Nihon University, Kanagawa, Japan. |
| |
| Abstract: | Yeast extract-treated suspension cultures of a new cell line, AK-1, of Glycyrrhiza echinata were induced to produce an isoflavonoid phytoalexin (medicarpin) and metabolites of retrochalcone/flavone pathway (echinatin, licodione, and 7,4'-dihydroxyflavone). From these cells, putative full-length cDNAs encoding cytochrome P450s, (2S)-flavanone 2-hydroxylase and isoflavone 2'-hydroxylase, were cloned. |
| |
| Keywords: | |
|
|
