Characteristics of a newly created bioluminescent Pseudomonas putida harboring TOL plasmid for use in analysis of a bioaugmentation system |
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| Authors: | Xin-Hui Xing Takakuni Tanaka Kanji Matsumoto Hajime Unno |
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| Institution: | (1) Division of Material Science and Chemical Engineering, Yokohama National University, Yokohama, 240-8501, Japan;(2) Department of Bioengineering, School of Life Sciences and Technology, Tokyo Institute of Technology, Yokohama, 226, Japan |
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| Abstract: | Insertion of a bacterial lux operon into the chromosome of Pseudomonas putida mt-2 holding TOL plasmid, yielded a new bioluminescent strain of P. putida BLU. Both in the cultures containing toluene and m-toluic acid as the sole carbon sources, P. putida BLU showed the same specific growth rate and cell yield as those of the wild strain. The bioluminescence output in the cell growth phases correlated with the cell concentration, indicating that the bioluminescent P. putida BLU can be monitored and quantified in a mixed culture in real time by the luminescence detection. |
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| Keywords: | bioaugmentation bioluminescence Pseudomonas putida TOL plasmid xenobiotics |
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