Morphological and molecular characterization of a natural hybrid betweenOrchis laxiflora andO. morio (Orchidaceae) |
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| Authors: | P Caputo S Aceto S Cozzolino R Nazzaro |
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| Institution: | (1) Dipartimento di Biologia Vegetale, Universitá di Napoli  Federico II , Via Foria, 223, I-80139 Napoli, Italy;(2) Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli Federico II, Via Mezzocannone, 8, I-80134 Napoli, Italy;(3) Orto Botanico, Università di Napoli Federico II, Via Foria, 223, I-80139 Napoli, Italy;(4) Dipartimento di Biologia Vegetale, Universitá di Napoli Federico II, Via Foria, 223, I-80139 Napoli, Italy |
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| Abstract: | A putative natural hybrid betweenOrchis laxiflora andO. morio (Orchidaceae) from southern Italy, formerly known asO. alata, was characterized both on morphological and molecular grounds in order to confirm its hybrid status and to trace its maternal lineage. The morphological characters of the putative hybrid showed intermediacy between those of the parent species, and restriction fragment length polymorphism (RFLP) analysis of a region of the nuclear ribosomal DNA confirmed its hybrid origin. Chloroplast DNA RFLP analysis indicated thatO. morio provided the maternal genome. |
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| Keywords: | Orchidaceae Orchis Orchis × alata Hybridization plastid heredity |
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