Molecular organisation of the quinic acid utilization (QUT) gene cluster in Aspergillus nidulans |
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| Authors: | Alastair R Hawkins Heather K Lamb Melanie Smith John W Keyte and Clive F Roberts |
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| Institution: | (1) Department of Genetics, University of Newcastle upon Tyne, NE2 4HH Newcastle upon Tyne, UK;(2) Department of Biochemistry, University of Leicester, LE1 7RH Leicester, UK;(3) Department of Genetics, University of Leicester, LE1 7RH Leicester, UK |
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| Abstract: | Summary The functional integrity of the QUTB gene (encoding quinate dehydrogenase) has been confirmed by transformation of a qutB mutant strain. The DNA sequence of the contiguous genes QUTD (quinate permease), QUTB and QUTG (function unknown) has been determined and analysed, together with that of QUTE (catabolic 3-dehydroquinase). The QUTB sequence shows significant homology with the shikimate dehydrogenase function of the complex AROM locus of Aspergillus nidulans, and with the QA-3 quinate dehydrogenase and QA-1S (repressor) genes of Neurospora crassa. The QUTD gene shows strong homology with the N. crassa QA-Y gene and QUTG with the QA-X gene. QUTD, QUTB, and QUTG, QUTE form two pairs of divergently transcribed genes, and conserved sequence motifs identified in the two common 5 non-coding regions show significant homology with UAS
GAL
and UAS
QA
sequences of the Saccharomyces cerevisiae and N. crassa Gal and QA systems. In addition, conserved 5 sequences homologous to the mammalian CAAT box are noted and a previously unreported conserved 22 nucleotide motif is presented. |
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