MicroRNA-221 regulates osteosarcoma cell proliferation,apoptosis, migration,and invasion by targeting CDKN1B/p27 |
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Authors: | Xiao-Hui Hu Ze-Xue Zhao Jian Dai De-Chun Geng Yao-Zeng Xu |
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Institution: | 1. Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, China
Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China;2. Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China;3. Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, China |
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Abstract: | MicroRNAs (miRNAs, miR) are of critical importance in growth and metastasis of cancer cells; however, the underlying functions of miRNAs in osteosarcoma (OS) remain largely unknown. This study was aimed to elucidate the role of miR-221 in regulating the biological behavior of OS cells. The proliferation ability was examined by cell counting kit-8 (CCK-8) and cell cycle assay. The abilities of cell migration, invasion, and apoptosis were monitored by transwell assay and flow cytometry, respectively. The effect of miR-221 on cyclin-dependent kinase inhibitor 1B (CDKN1B) expression was evaluated by luciferase assays, real-time polymerase chain reaction, and Western blot analysis. We found that miR-221 was elevated in OS cell lines compared with the normal osteoblastic cell line. Transfection of the miR-221 inhibitor into MG63 and U-2OS cell lines obviously suppressed cell proliferation, migration, and invasion, which is accompanied with cell cycle arrest in G0/G1 phase. Furthermore, luciferase reporter assays indicated that CDKN1B is directly targeted by miR-221 in OS cells. Knockdown of CDKN1B inhibited the effects of miR-221 inhibitor, along with decreased Bax and caspase-3 and increased cyclin E, cyclin D1, Bcl-2, Snail, and Twist1 expression. The results suggested that miR-221 might act as a potentially useful target for treatment of OS. |
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Keywords: | cyclin-dependent kinase inhibitor 1B (CDKN1B) invasion microRNA (miRNA)-221 migration proliferation |
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