Incorporated-bFGF polycaprolactone/polyvinylidene fluoride nanocomposite scaffold promotes human induced pluripotent stem cells osteogenic differentiation |
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| Authors: | Mohammad Foad Abazari Fatemeh Soleimanifar Seyed Ehsan Enderami Mahsa Nematzadeh Navid Nasiri Fatemeh Nejati Ehsan Saburi Shabanali Khodashenas Behzad Darbasizadeh Mohammad Mehdi Khani Pegah Ghoraeian |
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| Institution: | 1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran;2. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran;3. Molecular and Cell biology Research Center, Faculty of Medicine and Thalassemia Research Center, Hemoglobinopathy Institute, 4. Mazandaran University of Medical Sciences, Sari, Iran;5. Young Researchers and Elit Club, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran;6. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran;7. Immunogenetics and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;8. Department of Medical Biotechnology, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;9. Department of Pharmaceutics and Pharmaceutical Nanotechnology, Student Research Committee, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran;10. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;11. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran |
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| Abstract: | Bioactive scaffolds that can increase transplanted cell survival time at the defect site have a great promising potential to use clinically since tissue regeneration or secretions crucially depend on the transplanted cell survival. In this study embedded basic fibroblast growth factor (bFGF)-polycaprolactone-polyvinylidene fluoride (PCL-PVDF) hybrid was designed and fabricated by electrospinning as a bio-functional nanofibrous scaffold for bone tissue engineering. After morphological characterization of the PCL-PVDF (bFGF) scaffold, nanofibers biocompatibility was investigated by culturing of the human induced pluripotent stem cells (iPSCs). Then, the bone differentiation capacity of the iPSCs was evaluated when grown on the PCL-PVDF and PCL-PVDF (bFGF) scaffolds in comparison with culture plate as a control using evaluating of the common osteogenic markers. The viability assay displayed a significant increase in iPSCs survival rate when grown on the bFGF content scaffold. The highest alkaline phosphatase activity and mineralization were detected in the iPSCs while grown on the PCL-PVDF (bFGF) scaffolds. Obtained results from gene and protein expression were also demonstrated the higher osteoinductive property of the bFGF content scaffold compared with the scaffold without it. According to the results, the release of bFGF from PCL-PVDF nanofibers increased survival and proliferation rate of the iPSCs, which followed by an increase in its osteogenic differentiation potential. Taking together, PCL-PVDF (bFGF) nanofibrous scaffold demonstrated that can be noted as a promising candidate for treating the bone lesions by tissue engineering products. |
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| Keywords: | basic fibroblast growth factor nanofibers osteogenesis polycaprolactone polyvinylidene fluoride |
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