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Overexpression of Axl reverses endothelial cells dysfunction in high glucose and hypoxia
Authors:Zuo Pei-Yuan  Liu Yu-Wei  Zha Xiang-Nan  Tong Song  Zhang Rong  He Xiao-Xiao  Shan Sheng-Shuai  Wang Kun  Liu Cheng-Yun
Affiliation:1. Department of Geriatrics, Tongji Hospital Affiliated to The Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China;2. Department of Geriatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China;3. Department of Geriatrics, Beijing Hospital, Beijing, People's Republic of China;4. Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China;5. Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China;6. Department of Gastroenterology, Liyuan Hospital Affiliated to The Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China;7. Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China
Abstract:
The receptor tyrosine kinase Axl is involved in diabetic vascular disease. This study aims to investigate the effect of high glucose on endothelial cells injury and Axl expression in hypoxia condition in vitro, and we present details of the mechanism associated with overexpression of Axl rescue the high glucose injury. Our results showed that high glucose impaired both human umbilical vein endothelial cells (HUVECs) and EAhy926 cells angiogenesis in hypoxia condition. In addition, high glucose inhibits Axl and hypoxia-inducible factor 1-α (HIF-1α) protein expression in hypoxia condition. Axl overexpression significantly reversed endothelial cells dysfunction in high glucose/hypoxia. Furthermore, Axl overexpression in EAhy926 cells increases HIF-1α protein synthesis through PI3K/Akt/mTOR/p70 S6K signal pathway but not Mek/Erk in high glucose/hypoxia condition. This study demonstrates that high glucose can alter Axl signaling and HIF-1α in hypoxia condition. Overexpression of Axl may rescue endothelial cells dysfunction and HIF-1α expression through its downstream signals in high glucose/hypoxia.
Keywords:angiogenesis  Axl  endothelial cell  high glucose  hypoxia
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