| 流式细胞仪检测中细胞DNA样品制备的探讨 |
| |
| 引用本文： | 林珏龙,洪超群,朴英姬,陈耀文,霍霞.流式细胞仪检测中细胞DNA样品制备的探讨[J].激光生物学报,2002,11(2):122-125. |
| |
| 作者姓名： | 林珏龙 洪超群 朴英姬 陈耀文 霍霞 |
| |
| 作者单位： | 1. 汕头大学医学院中心实验室,中国广东,汕头,515031
2. 汕头大学医学院肿瘤医院,中国广东,汕头,515031 |
| |
| 摘 要： | 为了获得适合流式细胞仪检测的样品，通过碘化同啶标记和流式细胞仪对食管癌细胞内DNA含量分布进行分析，在不同的时间内对血液中淋巴细胞内DNA含量的测定结果进行研究。结果显示：结构完整的细胞DNA的荧光点图形成二倍体，四倍体的区域。组A各样品具有良好一致性，1～3天内差异小；组B各样品差异较大，因此细胞的完整性是DNA含量检测的基本条件，细胞经PI染色后若不能及时检测，可避光、4℃放置1～3天仍可获得较理想的实验结果。
|
| 关 键 词： | 流式细胞仪 检测 细胞DNA 样品制备 |
| 文章编号： | 1007-7146(2002)02-0122-04 |
| 收稿时间： | 2001/12/4 |
| 修稿时间： | 2001年12月4日 |
Study of Sample Preparation of Cellular DNA in Test of Flow Cytometry |
| |
| LIN Jue-long ,HONG Chao-qun ,PIAO Ying-ji ,CHEN Yao-wen ,HUO Xa.Study of Sample Preparation of Cellular DNA in Test of Flow Cytometry[J].ACTA Laser Biology Sinica,2002,11(2):122-125. |
| |
| Authors: | LIN Jue-long HONG Chao-qun PIAO Ying-ji CHEN Yao-wen HUO Xa |
| |
| Institution: | LIN Jue-long 1,HONG Chao-qun 2,PIAO Ying-ji 1,CHEN Yao-wen 1,HUO Xa 1 |
| |
| Abstract: | To develop a proper sample preparation method for flow cytometry. Using propidium iodide labeling and flow cytometry, we analyzed distributive image of DNA content in cell of esophageal carcinoma and compared DNA content of lymphocytes in blood during difference times. Dot plot image of DNA fluorescence could form region of diploid and tetraploid in integrated cell. All samples of group A had a good consistent and was little difference in three days, but that of group B had a great diversity. Cellular integrity is basic condition of measurement on DNA content. After propidium iodide labeling, if cell is not tested on time, it could be keeped away from beam and stored at 4℃. We can get good result in three days. |
| |
| Keywords: | flow cytometry DNA content propidium iodide sample preparation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
